US 8,273,310 B2 · Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device

Abstract

A centrifugal force-based microfluidic device for nucleic acid extraction and a microfluidic system are provided. The microfluidic device includes a body of revolution; a microfluidic structure disposed in the body of revolution, the microfluidic structure including a plurality of chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structure transmitting the fluid using centrifugal force due to rotation of the body of revolution; and magnetic beads contained in one of the chambers which collect a target material from a biomaterial sample flowing into the chamber, wherein the microfluidic structure washes the magnetic beads which collect the target material, and separates nucleic acid by electromagnetic wave irradiation from an external energy source to the magnetic beads. The microfluidic system includes the microfluidic device; a rotation operating unit which rotates the body of revolution; and an external energy source which irradiates electromagnetic waves.

Claims20

Dependent claims

A centrifugal force-based microfluidic device for extracting a micro element from a material sample, comprising:

a body of revolution;
at least one microfluidic structure disposed in the body of revolution,
wherein the microfluidic structure comprises:
a plurality of chambers;
a plurality of channels which connect the chambers;
and
a plurality of valves which are disposed in the channels to control fluid flow, and
wherein the microfluidic structure transmits fluid using centrifugal force due to rotational movement of the body of revolution;
and
micro-particles contained in one of the chambers which capture a target material from the material sample flowing into the chamber,
an external energy source configured to irradiate electromagnetic waves onto the micro-particles having the target material,
wherein the microfluidic structure is configured to wash the micro-particles having the target material in the chamber, and to separate the micro element from the target material in another chamber by the electromagnetic wave.

9 more elements

A microfluidic system comprising:

a microfluidic device comprising:
a body of revolution;
at least one microfluidic structure disposed in the body of revolution,
wherein the microfluidic structure comprises:
a plurality of chambers;
a plurality of channels which connect the chambers;
and
a plurality of valves which are disposed in the channels and control fluid flow, and
wherein the microfluidic structure transmits fluid using centrifugal force due to rotational movement of the body of revolution;
and
micro-particles contained in one of the chambers which capture a target material from a material sample flowing into the chamber;
a rotation operating unit which rotates the body of revolution of the microfluidic device;
and
an external energy source configured to irradiate electromagnetic waves onto the micro-particles having the target material,
wherein the microfluidic structure is configured to wash the micro-particles having the target material, and to separate a micro element from the target material in another chamber by the electromagnetic wave.

12 more elements

Dependencies are inferred from the claim text.

Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device

Life of this patent

Centrifugal force-based microfluidic device for nucleic acid extraction and microfluidic system including the microfluidic device

Life of this patent

Abstract

Claims20