Method for recombinant microorganism expression and isolation of collagen-like polypeptides

A process for producing a collagen-like polypeptide, wherein said process comprises the following steps:

• (a) expressing a nucleic acid sequence encoding said collagen-like polypeptide in a fungus host;
• (b) growing said fungus in culture medium;
• and
• (c) purifying said collagen-like polypeptide from said culture medium;
• wherein said collagen-like polypeptide comprises consecutive [Gly-X-Y] repeats;
• wherein Gly stands for glycine, X and Y represent any amino acid;
• wherein the length of the consecutive [Gly-X-Y] repeat being such that its weight on amino acid basis is at least 2.5 kDa;
• wherein the amino acid sequence of said collagen-like polypeptide comprises more than 4 different amino acids;
• and wherein said collagen-like polypeptide is free of all the sequences LXXR (SEQ ID NO:51), IXXR (SEQ ID NO:52), VXXR (SEQ ID NO:53), MXXR (SEQ ID NO:54), and MGPR (SEQ ID NO:14), and wherein said purified collagen-like polypeptide is free of helix structure.