Methods and materials for conferring resistance to pests and pathogens of plants
AssigneeVenganza Inc.
InventorCharles L. Niblett
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Abstract
Methods and materials for conferring pest resistance to plants are provided. Plants are transformed with a silencing construct homologous to a gene of a plant pest that is essential for the survival, development, or pathogenicity of the pest. This results in the plant producing RNAi to the selected gene, which, when ingested by the pest results in silencing of the gene and a subsequent reduction of the pest's ability to harm the plant. In other embodiments, the pest's reduced ability to harm the plant is passed on to pest progeny. Methods and materials for depathogenesis of pests is also provided.
Claims10
A method of conferring fungal resistance to a plant comprising expressing in the plant:
- (a) an antisense sequence having at least 20 contiguous nucleotides of at least one sequence selected from the group consisting of SEQ ID NOs:
- 8, 43, 44, 46, 50, 54, 55, 58, 59 and 65;
- (b) a sense sequence substantially complementary to the antisense sequence, wherein a transcript of the antisense sequence and a transcript of the sense sequence are capable of hybridizing to form a double-stranded RNA;
- and whereby the plant expressing said double stranded RNA is resistant to a fungus expressing at least one ribosomal RNA gene having substantial homology to the antisense sequence.
A transgenic plant that is resistant to fungus, the transgenic plant comprising:
- (a) an antisense sequence having at least 20 contiguous nucleotides of at least one sequence selected from the group consisting of SEQ ID NOs:
- 8, 43, 44, 46, 50, 54, 55, 58, 59 and 65;
- (b) a sense sequence substantially complementary to the antisense sequence, wherein a transcript of the antisense sequence and a transcript of the sense sequence are capable of hybridizing to form a double-stranded RNA;
- and
- whereby the plant expressing said double-stranded RNA is resistant to a fungus expressing at least one ribosomal RNA gene having substantial homology to the antisense sequence.
Dependencies are inferred from the claim text.