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US 8,148,515 B1Granted
Detection using a dye and a dye modifier
AssigneeBiotium, Inc.
InventorsFei Mao, Wai-Yee Leung, Hye Eun Hoover
Life of this patent
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Abstract
The present invention relates to dyes in general. The present invention provides a wide range of dyes and kits containing the same, which are applicable for labeling a variety of biomolecules such as nucleic acids, cells and microorganisms. The present invention also provides various methods of using the dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions.
Claims14
A reagent composition comprising:
- (a) a light-emitting dye capable of binding to nucleic acid, wherein the light-emitting dye is substantially non-fluorescent when uncomplexed with said nucleic acid in the absence of a light-emitting dye modifier;
- and
- (b) a light-emitting dye modifier comprises at least two sulfonate groups that is not covalently bonded to the light-emitting dye;
- wherein the reagent composition permits detection of nucleic acid present in a concentration that is lower than minimum concentration detectable using identical detection means and another reagent composition, said another reagent composition having the light-emitting dye present in the same concentration but lacking said modifier.
1 more element
Dependencies are inferred from the claim text.