hTERT gene expression regulatory gene
AssigneeNational University Corporation Tottori University
InventorNorimasa Miura
Life of this patent
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Abstract
Disclosed is a novel substance capable of regulating the expression of a telomerase reverse transcriptase gene in a cell of a mammal. A gene capable of regulating the expression of hTERT, comprising a nucleotide sequence depicted in SEQ ID No: 1 or 2. The expression of a telomerase reverse transcriptase gene can be inhibited by inhibiting the expression of the gene. By utilizing this mechanism, the expression of a telomerase reverse transcriptase gene can be regulated.
Claims15
A double stranded RNA comprising:
- a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA;
- and
- a second base sequence with a length of 15 bases or longer and 30 bases or shorter, complementary to the first base sequence;
- wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) the base sequence shown in SEQ ID No. 4;
- and
- (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4;
- and wherein the second base sequence is sufficiently complementary to the hTERT expression regulatory RNA to suppress expression of the hTERT expression regulatory RNA.
A single stranded RNA comprising:
- a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA;
- and
- a second base sequence with a length of 15 bases or longer and 30 bases or shorter, disposed in the opposite direction to the first base sequence, and complementary to the first base sequence;
- wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) the base sequence shown in SEQ ID No. 4;
- and
- (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4;
- and wherein the second base sequence is sufficiently complementary to the hTERT expression regulatory RNA to suppress expression of the hTERT expression regulatory RNA.
An hTERT expression regulatory gene expression vector, comprising a DNA sequence and a vector, wherein the DNA sequence encodes a single stranded RNA that consists of:
- a first base sequence with a length of 15 bases or longer and 30 bases or shorter, corresponding to one portion of an hTERT expression regulatory RNA;
- a second base sequence with a length of 15 bases or longer and 30 bases or shorter, disposed in the opposite direction to the first base sequence, and complementary to the first base sequence;
- and
- a hairpin loop sequence of 4 bases or more;
- wherein the hairpin loop sequence is located between the first base sequence and the second base sequence;
- and
- the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) the base sequence shown in SEQ ID No. 4;
- and
- (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4;
- and wherein the second base sequence is sufficiently complementary to the hTERT expression regulatory RNA to allow the single stranded RNA to suppress expression of the hTERT expression regulatory RNA.
An RNA composition comprising a plurality of types of double stranded RNA formed by cleaving, with a micro RNA maturation enzyme, a precursor double stranded RNA that comprises:
- an hTERT expression regulatory RNA, and
- an RNA comprising a base sequence complementary to the hTERT expression regulatory RNA,
- wherein the hTERT expression regulatory RNA comprises a member selected from the group consisting of:
- (a) the base sequence shown in SEQ ID No. 4;
- and
- (b) a base sequence wherein one base is deleted from, replaced in, or added to the base sequence shown in SEQ ID No. 4.
Dependencies are inferred from the claim text.