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AssigneeThe University of North Carolina at Chapel Hill
InventorsRachel T. Noble, Angelia D. Blackwood
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The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NO:1-4, the novel oligonucleotide probe sequences set forth in SEQ ID NO:5-8, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly E. coli and Enterococcus spp. in a sample.
A composition for the detection of a multiplicity of strains and species of fecal indicator bacteria in an environmental sample, wherein said composition comprises, in the 5′ to 3′ direction, one of a fluorophore or a quencher molecule, an oligonucleotide probe comprising SEQ ID NO:5 or 7, the other of the fluorophore or quencher molecule, a PCR blocker moiety, and an oligonucleotide comprising SEQ ID NO:1, wherein a portion of the oligonucleotide probe SEQ ID NO:5 or 7 binds to an amplification product of SEQ ID NO:1.
A composition for the detection of a multiplicity of strains and species of fecal indicator bacteria in an environmental sample, wherein said composition comprises, in the 5′ to 3′ direction, one of a fluorophore or a quencher molecule, an oligonucleotide probe comprising SEQ ID NO:6 or 8, the other of the fluorophore or quencher molecule, a PCR blocker moiety, and an oligonucleotide comprising SEQ ID NO:3 wherein a portion of the oligonucleotide probe SEQ ID NO:6 or 8 binds to an amplification product of SEQ ID NO:3.
A method for detecting the presence of a multiplicity of strains and species of fecal indicator bacteria in an environmental sample using polymerase-based amplification of a target nucleic acid region present in said fecal indicator bacteria, said method comprising:
A method for detecting the presence of a multiplicity of strains and species of fecal indicator bacteria in an environmental sample using polymerase-based amplification of a target nucleic acid region present in said fecal indicator bacteria, said method comprising:
Dependencies are inferred from the claim text.