Protein kinase-inducible domains
AssigneeUniversity of Delaware
InventorsNeal J. Zondlo, Shalini Balakrishnan
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Abstract
Applicants have used protein design to develop novel functional protein architectures, termed protein kinase-inducible domains, whose structures are dependent on phosphorylation by specific protein kinases or are dependent on dephosphorylation by specific protein phosphatases. Applicants have designed kinase-inducible domains based on a modular architecture, which allows kinase-inducible domains to be responsive to any specific serine-threonine kinases. Kinase-inducible domains can consist of canonical amino acids, allowing their use as expressible tags of protein kinase activity.
Claims6
An isolated polynucleotide which encodes a polypeptide comprising an EF hand domain, wherein the EF hand domain has a structurally significant glutamic acid or aspartic acid residue substituted with a serine or threonine residue, wherein the EF hand domain has the formula:
- X1X2X3X4X5X6X7X8X9X10X11X12X13X14X15X16
- wherein X1 is an optionally present amino acid;
- X2 is an optionally present amino acid;
- X3 is K, A, or L;
- X4 is N or D;
- X5 is A or G;
- X6 is G or R;
- X7 is I or R;
- X8 is D, R, S, or T;
- X9 is A, R, K, P, or F;
- X10 is an optionally present A, R, G, or L, provided that, when X8 is not S or T, X10 is present;
- X11 is an optionally present S, T, or R, provided that, when X8 is not S or T, X″ is present and is S or T;
- X12 is an optionally present L, P, I, K, or F;
- X13 is an optionally present K, A, I, R, G, or L;
- X14 is an optionally present A, R, or P;
- X15 is an optionally present K;
- X16 is an optionally present A.
Dependencies are inferred from the claim text.