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US 8,148,496 B2Granted
Multivalent antibody constructs
AssigneeDeutsches Krebsforschungszentrum
InventorsMelvyn Little, Sergej Kipriyanov
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Abstract
The present invention relates to a multivalent Fv antibody construct having at least four variable domains which are linked with each over via the peptide linkers 1, 2 and 3. The invention also concerns expression plasmids which code for such an Fv antibody construct and a method of producing the Fv antibody constructs as well as their use.
Claims6
A monospecific, homodimeric and tetravalent Fv antibody formed by two single-chain Fv monomers of the same kind, each of the single-chain Fv monomer having the structure:
- VH-peptide linker 1-VL-peptide linker 2-VH-peptide linker 3-VL, wherein
- with the proviso that each Fv monomer does not have constant domains;
- VH and VL are heavy chain and light chain variable domains of an antibody specific for an antigen, respectively;
- peptide linker 1 is a peptide bond or has 1 to about 10 amino acids;
- peptide linker 2 has about 3 to 10 amino acids;
- peptide linker 3 is a peptide bond or has 1 to about 10 amino acids;
- and
- each VH of one of the single-chain Fv monomers associates intermolecularly with each VL of the other single-chain Fv monomer, thereby forming said homodimeric and tetravalent Fv antibody having four antigen binding sites for the same antigen.
5 more elements
Dependencies are inferred from the claim text.