Composition and method for in vivo and in vitro attenuation of gene expression using double stranded RNA
AssigneeGeorgia Health Sciences University Research Institute, Inc.
InventorsYin-Xiong Li, Michael J. Farrell, Margaret J. Kirby
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Abstract
Introduction of double stranded RNA into cells, cell culture, organs and tissues, and whole organisms, particularly vertebrates, specifically attenuates gene expression.
Claims27
A method for attenuating the expression of a target gene in a cell, the method comprising contacting an embryonic zebrafish, embryonic chick or fibroblast murine cell with a double stranded RNA (dsRNA) in an amount sufficient to specifically attenuate expression of the target gene, wherein one of the strands of the double stranded RNA is capable of hybridizing to the target gene in vitro in 400 mM NaCl, 40 mM PIPES pH 6.4, and 1 mM EDTA, at 50° C.
Dependencies are inferred from the claim text.