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US 8,148,172 B2Granted
Methods for ionophorically screening pore forming bacterial protein toxins and receptors
OwnerJohn Cuppolettiinventor
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Abstract
One embodiment of the present invention is directed to methods for ionophorically screening pore forming bacterial protein toxins and receptors. The method includes: a) forming a membrane comprising a lipid and a receptor, b) contacting the membrane with the pore forming bacterial protein toxin and an ion solution, and c) measuring ion flow through the membrane.
Claims9
A method for ionophorically screening pore forming bacterial protein toxins and receptors, comprising:
- a) forming a membrane in a receptacle comprising an aperture by combining, a lipid bilayer and a receptor, such that the membrane forms across the aperture and provides separation between two compartments in the receptacle, wherein the lipid bilayer comprises at least one of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), 1-palmitoyl-2, oleoyl-sn-glycero-3-phosphatidylserine (POPS), 1,2-diphytanoyl-sn-glycero-3-phosphocholine, and cholesterol;
- b) by contacting the membrane with the pore forming bacterial protein toxin and an ion solution, whereby the pore forming bacterial protein toxin binds the receptor and forms a pore in the membrane;
- and
- c) measuring ion flow through the pore in the membrane.
1 more element
Dependencies are inferred from the claim text.