Method and device for extracting an analyte
AssigneePhyNexus, Inc.
InventorsDouglas T. Gjerde, Ronald Jones, Allen Burge
Life of this patent
Estimating the term…
Abstract
The invention provides extraction columns for the purification of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution, as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. In some embodiments, the method involves adjusting the head pressure of the column during the process, or otherwise controlling or regulating the head pressure.
Claims10
An automated parallel method for passing liquid through a plurality of pipette tip columns comprising the steps of:
- a) providing a plurality of pipette tip columns, wherein each column is comprised of:
- i. a column body having an open upper end for communication with a pump, a first open lower end for the uptake and dispensing of fluid, and an open passageway between the upper and lower ends of the column body,
- ii. a bottom frit, wherein the bottom frit is at or near the open lower end of the pipette tip column,
- iii. an optional top frit positioned between the bottom frit and the open upper end of the column body,
- iv. a bed of hydrated gel resin media,
- v. a storage liquid, wherein the storage liquid is comprised of a water miscible solvent having a viscosity greater than that of water, and
- vi. a pump sealingly attached to the open upper end of the column, wherein the pump is comprised of a piston, and wherein actuation of the pump piston causes liquid to be drawn into or expelled from the bed of hydrated gel resin media;
- b) contacting said open lower ends of the columns with a liquid;
- c) actuating the pump piston to draw the liquid into the open lower ends of the columns and through the bed of hydrated gel resin media, wherein the volume of liquid drawn into the columns is 250 μl or less and wherein the pump piston displacement is 20 to 75 μl greater than the volume of liquid drawn into the column;
- and
- d) actuating the pump piston to expel the liquid through the bed of hydrated gel resin media and out of the open lower end of the columns.
An automated parallel method for passing liquid through a plurality of pipette tip columns comprising the steps of:
- a) providing a plurality of pipette tip columns, wherein each column is comprised of:
- i. a column body having an open upper end for communication with a pump, a first open lower end for the uptake and dispensing of fluid, and an open passageway between the upper and lower ends of the column body,
- ii. a bottom frit, wherein the bottom frit is at or near the open lower end of the pipette tip column,
- iii. an optional top frit positioned between the bottom frit and the open upper end of the column body,
- iv. a bed of resin media, and
- v. a pump sealingly attached to the open upper end of the column, wherein the pump is comprised of a piston, and wherein actuation of the pump piston causes liquid to be drawn into or expelled from the bed of resin media;
- b) contacting said open lower ends of the columns with a liquid;
- c) actuating the pump piston to draw the liquid into the open lower ends of the columns and through the bed of resin media, wherein the volume of liquid drawn into the columns is 250 μl or less wherein the pump piston displacement is 20 to 75 μl greater than the volume of liquid drawn into the column;
- and
- d) actuating the pump piston to expel the liquid through the bed of resin media and out of the open lower end of the columns.
Dependencies are inferred from the claim text.