US 8,148,152 B2 · Method for the preparation of embryoid bodies (EBs) and uses thereof

Abstract

Provided are means and methods for producing embryoid bodies (EBs) from multi- or pluripotent cells. In particular, a method of generating embryoid bodies (EBs) is described comprising agitation of a liquid suspension culture of multi- or pluripotent cells in a container until generation of cell aggregates, optionally diluting the suspension, and further agitation of the suspension until formation of EBs. Furthermore, the present invention relates to the use of the novel culturing method and EBs obtained thereby for a variety of applications including genomics, diagnostic assays, teratogenic/embryotoxicological and pharmacological assays as well as for the provision of tissue grafts.

Claims48

Dependent claims

A method for producing embryoid bodies (EBs) from pluripotent cells comprising

(i) obtaining a liquid single cell suspension culture of pluripotent cells;
(ii) collecting and suspending the cells in a container to a density of about 0.5×106 to 5×106 cells/ml;
(iii) rocking the container containing the liquid single cell suspension culture thereby generating cell aggregates;
and
(iv) diluting the suspension, and further rocking a container containing the suspension until formation of EBs;
wherein the final concentration of EBs in the suspension culture is about 500 EBs/ml;
wherein the pluripotent cells are selected from the group consisting of embryonic stern (ES) cells, primordial germ (EG) cells and non-embryonic pluripotent stem cells.

4 more elements

Dependencies are inferred from the claim text.

Method for the preparation of embryoid bodies (EBs) and uses thereof

Life of this patent

Method for the preparation of embryoid bodies (EBs) and uses thereof

Life of this patent

Abstract

Claims48