Donor specific antibody libraries
AssigneeSea Lane Biotechnologies, LLC
InventorsLawrence Horowitz, Ramesh Bhatt, Arun K. Kashyap
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Abstract
The present invention concerns donor-specific antibody libraries derived from a patient donor who has suffered from, or is suffering from one or more diseases discussed herein. The present invention also concerns the method of making and using the donor-specific antibodies. The present invention further concerns the neutralizing antibodies obtained from the donor-specific antibody libraries and the methods of using these antibodies for the prevention/treatment of human disease.
Claims18
A method of making a donor-specific library expressing a collection of antibodies or antibody fragments to a target antigen, comprising the steps of:
- a) obtaining mRNA from lymphocytes of a human patient donor who has suffered from, or who is suffering from a disease evoking antibody production to said antigen;
- b) generating in a vector a collection of nucleic acids comprising sequences encoding an immunoglobulin repertoire of said patient by reverse transcription of said obtained mRNA;
- and
- c) identifying said donor-specific library with a unique nucleotide sequence barcode that is linked to and labels said nucleic acids in the vector.
Dependencies are inferred from the claim text.