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US 8,148,071 B2Granted
Methods and compositions for isolating nucleic acid
AssigneeNorthwestern University
InventorsDavid M. Kelso, Sujit Jangam, Douglas Yamada, Sally McFall
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Abstract
The present invention relates to compositions and methods for isolating and purifying nucleic acid. In particular, the present invention relates to methods of isolating nucleic acid from cells for use in further analysis.
Claims27
A method of processing biological samples, comprising:
- a) contacting with a blood sample at least one sample processing device comprising
- (i) a sample collection membrane with a pore size configured to retain nucleic acid and allow lysed red blood cells to flow through, and
- (ii) a blotter material in physical contact with said membrane;
- and
- b) contacting said membrane with a buffer under conditions such that said buffer lyses red blood cells, removes debris, and leaves purified white blood cells or components thereof on said membrane.
2 more elements
A biological sample processing kit, comprising:
- a) at least one sample processing device comprising
- (i) a sample collection membrane with a pore size configured to retain nucleic acid and allow lysed red blood cells to flow through and
- (ii) a blotter material in physical contact with said membrane;
- and
- b) a buffer configured to lyse red blood cells retained on said membrane.
2 more elements
Dependencies are inferred from the claim text.