Back to results
US 8,148,064 B2Granted
Labelled nucleotides
AssigneeIllumina Cambridge Limited
InventorsShankar Balasubramanian, Colin Barnes, Xiaohai Liu, John Milton
Life of this patent
Estimating the term…
Abstract
Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
Claims17
A method for detecting the extension of a primer annealed to a target polynucleotide, comprising:
- (a) providing at least one nucleoside comprising
- (1) a label attached via a first linker to the nucleoside base, and
- (2) a protecting group attached via a second linker to the 2′ or 3′ oxygen of the nucleoside sugar,
- wherein the first and second linkers are both cleavable under a single set of conditions;
- (b) extending the annealed primer with the nucleoside only if the nucleoside base is complementary to the base at the corresponding position of the target polynucleotide;
- (c) detecting the presence of the label on the extended primer;
- and
- (d) incubating the extended primer under the single set of cleavage conditions, whereby both the first and second linkers are cleaved;
- thereby detecting the extension of the primer.
6 more elements
Dependencies are inferred from the claim text.