Enhanced production of fatty acid derivatives
AssigneeLS9, Inc.
InventorsZhihao Hu, Fernando Valle
Life of this patent
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Abstract
Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.
Claims33
A cultured recombinant cell, said cell comprising, (a) at least one gene encoding a fatty acid derivative enzyme selected from an acyl-CoA synthase (EC 2.3.1.86), a thioesterase (EC 3.1.2.- or EC 3.1.1.5), an acyl-CoA reductase (EC 1.2.1.50), a fatty alcohol forming acyl-CoA reductase (EC 1,1.1.*), and an ester synthase (EC 2.3.1.20 or EC 2.3.1.75), which gene is over-expressed, and (b) a gene encoding an acyl-CoA dehydrogenase enzyme (EC 1.3.99.3), wherein expression of the gene encoding said acyl-CoA dehydrogenase enzyme is attenuated, and wherein when cultured in the presence of a carbon source said cultured recombinant cell produces a fatty acid derivative selected from a fatty acid, a fatty aldehyde, a fatty alcohol and a fatty ester.
A recombinant cell culture comprising a recombinant cell, wherein one or more genes encoding a fatty acid derivative enzyme selected from a thioesterase (EC 3.1.2.- or EC 3.1.1.5), an ester synthase (EC 2.3.1.20 or EC 2.3.1.75) and an acyl-CoA synthase (EC 2.3.1.86) is over-expressed in the recombinant cell, and expression of a gene encoding an acyl-CoA dehydrogenase (EC 1.3.99.3) is attenuated in the recombinant cell, and wherein a fatty acid derivative selected from a fatty acid, a fatty aldehyde, a fatty alcohol and a fatty ester is found in the supernatant of said recombinant cell culture.
A recombinant cell culture comprising a recombinant cell, wherein one or more genes encoding a fatty acid derivative enzyme selected from a thioesterase (EC 3.1.2.- or EC 3.1.1.5), an ester synthase (EC 2.3.1.20 or EC 2.3.1.75), an acyl-CoA reductase (EC1.2.1.50), a fatty alcohol forming acyl-CoA reductase (EC 1,1.1.*), and an acyl-CoA synthase (EC 2.3.1.86) is over-expressed in the recombinant cell, and expression of a gene encoding an acyl-CoA dehydrogenase (EC 1.3.99.3) is attenuated in the recombinant cell, and wherein a fatty acid derivative selected from a fatty acid, a fatty aldehyde, a fatty alcohol and a fatty ester is found in the supernatant of said recombinant cell culture.
Dependencies are inferred from the claim text.