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US 7,888,552 B2Granted
Use of non-agrobacterium bacterial species for plant transformation
AssigneeMonsanto Technology LLC
InventorsXudong Ye, Junjiang Shen, Edward Williams
Life of this patent
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Abstract
The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.
Claims24
A method for transforming a corn plant cell, comprising:
- (a) contacting at least a first corn plant cell with a Rhizobiales bacterium other than Agrobacterium sp, comprising:
- (i) a first nucleic acid comprising a vir gene region of a Ti plasmid wherein the vir gene region acts to introduce a nucleic acid of interest into the plant cell in a VirD2-dependent manner;
- and (ii) a second nucleic acid comprising one or more T-DNA border sequence(s) operably linked to a nucleic acid of interest;
- and
- (b) selecting at least a first corn plant cell transformed with the nucleic acid of interest.
2 more elements
A Rhizobia cell selected from the group consisting of:
- Rhizobium leguminosarum USDA2370, R. leguminosarum bv. trifolii USDA2048, leguminosarum bv. phaseoli USDA2668, Rhizobium etli USDA 9032, Sinorhizobium fredii USDA205, S. meliloti USDA 1002, and Mesorhizobium loti USDA3471, the cell comprising (i) a first nucleic acid comprising a vir gene region of a Ti plasmid wherein the vir gene region acts to introduce a nucleic acid coding for a sequence of interest into a plant cell in a VirD2-dependent manner;
- and (ii) a second nucleic acid comprising one or more T-DNA border sequence(s) operably linked to a nucleic acid coding for a sequence of interest.
Dependencies are inferred from the claim text.