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AssigneeBoehringer Ingelheim Vetmedica GmbH
InventorsMarc Eichmeyer, Greg Nitzel, Merrill Schaeffer
Estimating the term…
An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time-consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
A kit for an immunogenic composition administered as a single dose immunogenic composition, said kit comprising:
A kit for an immunogenic composition administered as a single dose immunogenic composition, said kit comprising i) a container containing a single dose immunogenic composition, wherein said single dose immunogenic composition has 4 μg to 16 μg of recombinant PCV2 ORF2 protein per dose and a polymer of acrylic or methacrylic acid therein, and ii) a container containing an immunogenic composition comprising PRRS antigen.
A method for lessening the severity of clinical symptoms associated with PCV2 infection, said method comprising the step of administering a single dose of an immunogenic composition into pigs, wherein said immunogenic composition comprises 4 μg to 400 μg of recombinant PCV2 ORF2 protein per dose, a polymer of acrylic or methacrylic acid, and an immunogenic composition that comprises PRRS antigen, wherein the severity of clinical symptoms are lessened in pigs receiving said immunogenic composition in comparison to pigs not receiving said immunogenic composition and wherein said clinical symptoms are selected from the group consisting of wasting, paleness of the skin, unthriftiness, respiratory distress, diarrhea, icterus, jaundice, lung lesions, nasal shedding, cough, diarrhea and combinations thereof.
A method for lessening the severity of clinical symptoms associated with PCV2 infection, said method comprising the step of administering a single dose of an immunogenic composition into pigs, wherein said immunogenic composition comprises 4 μg to 100 μg of recombinant PCV2 ORF2 protein per dose, a polymer of acrylic or methacrylic acid, and an immunogenic composition that comprises PRRS antigen, wherein the severity of clinical symptoms are lessened in pigs receiving said immunogenic composition in comparison to pigs not receiving said immunogenic composition and wherein said clinical symptoms are selected from the group consisting of wasting, paleness of the skin, unthriftiness, respiratory distress, diarrhea, icterus, jaundice, lung lesions, nasal shedding, cough, diarrhea and combinations thereof.
A method for lessening the severity of clinical symptoms associated with PCV2 infection, said method comprising the step of administering a single dose of an immunogenic composition into pigs, wherein said immunogenic composition comprises 4 μg to 50 μg of recombinant PCV2 ORF2 protein per dose, a polymer of acrylic or methacrylic acid, and an immunogenic composition that comprises PRRS antigen, wherein the severity of clinical symptoms are lessened in pigs receiving said immunogenic composition in comparison to pigs not receiving said immunogenic composition and wherein said clinical symptoms are selected from the group consisting of wasting, paleness of the skin, unthriftiness, respiratory distress, diarrhea, icterus, jaundice, lung lesions, nasal shedding, cough, diarrhea and combinations thereof.
A method for lessening the severity of clinical symptoms associated with PCV2 infection, said method comprising the step of administering a single dose of an immunogenic composition into pigs, wherein said immunogenic composition comprises 4 μg to 16 μg of recombinant PCV2 ORF2 protein per dose, a polymer of acrylic or methacrylic acid, and a second immunogenic composition that comprises PRRS antigen, wherein the severity of one or more clinical symptoms are lessened in pigs receiving said single dose of said immunogenic composition in comparison to pigs not receiving said immunogenic composition and wherein said clinical symptoms are selected from the group consisting of wasting, paleness of the skin, unthriftiness, respiratory distress, diarrhea, icterus, jaundice, lung lesions, nasal shedding, cough, diarrhea and combinations thereof.
Dependencies are inferred from the claim text.