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US 7,767,800 B2Granted
Nucleic acids obtained from LAVMAL, a variant African AIDS virus
AssigneeInstitut Pasteur
InventorsMarc Alizon, Pierre Sonigo, Simon Wain-Hobson, Luc Montagnier
Life of this patent
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Abstract
A variant of a LAV virus, designated LAVMAL and capable of causing AIDS. The cDNA and antigens of the LAVMAL virus can be used for the diagnosis of AIDS and pre-AIDS.
Claims11
A purified RNA or DNA of HIV-1 encoding an HIV-1 polypeptide or containing an HIV-1 regulatory element and comprising an RNA or DNA that hybridizes under stringent conditions to at least one of the following cDNA fragments of LAVMAL:
- A. a sequence having nucleotides 1 to 96, which is the long terminal repeat R region of LAVMAL;
- B. a sequence having nucleotides 97 to 179, which is the 5′ long terminal repeat U5 region of LAVMAL;
- C. a sequence having nucleotides 8676 to 9133, which is the 3′ long terminal repeat U3 region of LAVMAL;
- D. a sequence having nucleotides 9134 to 9229, which is the 3′ long terminal repeat U3 region of LAVMAL;
- E. a sequence having nucleotides 5405 to 5620, which is the tat coding region of LAVMAL;
- F. a sequence having nucleotides 5134 to 5421, which is the vpr coding region of LAVMAL;
- G. a sequence having nucleotides 8380 to 9006, which is the nef coding region of LAVMAL;
- H. a sequence having nucleotides 350 to 1864, which is the gag coding region of LAVMAL;
- I. a sequence having nucleotides 1663 to 4668, which is the pol coding region of LAVMAL;
- J. a sequence having nucleotides 5799 to 8375, which is the env coding region of LAVMAL;
- K. a sequence having nucleotides 764 to 1501, which is the gag p25 coding region of LAVMAL;
- L. a sequence having nucleotides 1502 to 1864, which is the gag p13 coding region of LAVMAL;
- M. a sequence having nucleotides 5799 to 5885, which corresponds to amino acids 1-33 of the env coding region of LAVMAL;
- N. a sequence having nucleotides 5886 to 7337, which corresponds to amino acids 34 to 530 of the env coding region of LAVMAL;
- O. a sequence having nucleotides 5895 to 6176, which corresponds to amino acids 37 to 130 of the env coding region of LAVMAL;
- P. a sequence having nucleotides 6399 to 6635, which corresponds to amino acids 211 to 289 of the env coding region of LAVMAL;
- Q. a sequence having nucleotides 7212 to 7337, which corresponds to amino acids 488 to 530 of the env coding region of LAVMAL;
- R. a sequence having nucleotides 7215 to 7604, which corresponds to amino acids 490 to 620 of the env coding region of LAVMAL;
- and
- S. a sequence having nucleotides 7782 to 7844, which corresponds to amino acids 680 to 700 of the env coding region of LAVMAL,
- wherein the stringent hybridization conditions comprise hybridization in 50% formamide, 5×SSC at 42° C., and washing in 0.1×SSC, 0.1% SDS at 65° C. two times for 30 minutes, and wherein the RNA or DNA is not ARV2, and wherein the purified RNA or DNA of HIV-1 is not an RNA or DNA of LAVBRU.
18 more elements
A cDNA fragment of LAVMAL produced by the process comprising treating a cDNA sequence that comprises a nucleotide sequence of FIGS. 7A-7I with at least one restriction enzyme, wherein the restriction enzyme is selected from the group consisting of AvaI, BamHI, BqlII, EcoRI, HindIII, HincII, KpnI, NdeI, PstI, SacI, and XbaI.
A purified RNA or DNA of HIV-1, wherein the HIV-1 is LAVMAL or a variant of LAVMAL, comprising an RNA or DNA that hybridizes under stringent conditions to at least one of the following cDNA fragments of LAVMAL:
- A. a sequence having nucleotides 1 to 96, which is the long terminal repeat R region of LAVMAL;
- B. a sequence having nucleotides 97 to 179, which is the 5′ long terminal repeat U5 region of LAVMAL;
- C. a sequence having nucleotides 8676 to 9133, which is the 3′ long terminal repeat U3 region of LAVMAL;
- D. a sequence having nucleotides 9134 to 9229, which is the 3′ long terminal repeat U3 region of LAVMAL;
- E. a sequence having nucleotides 5405 to 5620, which is the tat coding region of LAVMAL;
- F. a sequence having nucleotides 5134 to 5421, which is the vpr coding region of LAVMAL;
- G. a sequence having nucleotides 8380 to 9006, which is the nef coding region of LAVMAL;
- H. a sequence having nucleotides 350 to 1864, which is the gag coding region of LAVMAL;
- I. a sequence having nucleotides 1663 to 4668, which is the pol coding region of LAVMAL;
- J. a sequence having nucleotides 5799 to 8375, which is the env coding region of LAVMAL;
- K. a sequence having nucleotides 764 to 1501, which is the gag p25 coding region of LAVMAL;
- L. a sequence having nucleotides 1502 to 1864, which is the gag p13 coding region of LAVMAL;
- M. a sequence having nucleotides 5799 to 5885, which corresponds to amino acids 1-33 of the env coding region of LAVMAL;
- N. a sequence having nucleotides 5886 to 7337, which corresponds to amino acids 34 to 530 of the env coding region of LAVMAL;
- O. a sequence having nucleotides 5895 to 6176, which corresponds to amino acids 37 to 130 of the env coding region of LAVMAL;
- P. a sequence having nucleotides 6399 to 6635, which corresponds to amino acids 211 to 289 of the env coding region of LAVMAL;
- Q. a sequence having nucleotides 7212 to 7337, which corresponds to amino acids 488 to 530 of the env coding region of LAVMAL;
- R. a sequence having nucleotides 7215 to 7604, which corresponds to amino acids 490 to 620 of the env coding region of LAVMAL;
- and
- S. a sequence having nucleotides 7782 to 7844, which corresponds to amino acids 680 to 700 of the env coding region of LAVMAL,
- wherein the stringent hybridization conditions comprise hybridization in 50% formamide, 5×SSC at 42° C., and washing in 0.1×SSC, 0.1% SDS at 65° C. two times for 30 minutes, and wherein the RNA or DNA is not ARV2, corresponds to either an ORF encoding polypeptides with the same immunogenic or immunological properties as the polypeptides of LAVMAL or to an HIV-1 regulatory element, and wherein the purified RNA or DNA of HIV-1 is not an RNA or DNA of LAVBRU.
18 more elements
Dependencies are inferred from the claim text.