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US 7,754,464 B2Granted
Cellular permissivity factor for viruses and uses thereof
AssigneePharmacia & Upjohn Company
InventorsJay Gregory Calvert, Shelly Shields, David E Slade, Siao-Kun Welch
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Abstract
The present invention provides methods and compositions related to the generation of host cells permissive for virus growth, particularly Porcine Reproductive and Respiratory Syndrome (PRRS) virus.
Claims16
A method of facilitating production of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) from a culture of vertebrate cells, comprising the steps of:
- (a) providing a recombinant vertebrate cell transfected with an exogenous polynucleotide that encodes a mammalian CD163 polypeptide having a transmembrane domain and at least 70% sequence identity to SEQ ID NO:14, so that expression of CD163 polypeptide in said cell is increased;
- (b) contacting a culture of said cell with PRRSV virus under conditions which permit infection of the cells and growth of the virus;
- and
- (c) recovering virus from said culture.
1 more element
Dependencies are inferred from the claim text.