Laser scanning microscope for fluorescence testing
AssigneeCarl Zeiss MicroImaging GmbH
InventorRalf Wolleschensky
Life of this patent
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Abstract
Laser scanning microscope for fluorescence testing, in which the illumination and detection rays are bound optically through a dichroic main beam splitter, the detected probe light being led to several detectors by means of a secondary beam splitter and the angle of incidence of the illumination light and/or the angle of incidence of the probe light at the splitter surface of at least the main beam splitter or at least the secondary splitter is less than 45 degrees.
Claims12
A laser scanning microscope for fluorescence testing of a specimen, the laser scanning microscope comprising:
- an optical axis:
- means for producing illumination rays along the optical axis in the direction of the specimen;
- means for producing detection rays from the specimen along the optical axis;
- a dichroic main beam splitter in the optical axis for optically guiding the illumination and detection rays, the main beam splitter having a surface;
- a plurality of detectors;
- and
- means for directing the detected rays to the plurality of detectors so that the angle of incidence of the illumination rays at the surface of the main beam splitter is less than 20 degrees.
A laser scanning microscope for fluorescence testing of a specimen, the laser scanning microscope comprising:
- an optical axis;
- means for producing illumination rays along the optical axis in the direction of the specimen;
- means for producing detection rays from the specimen along the optical axis;
- a dichroic secondary beam splitter in the optical axis for optically guiding the illumination and detection rays;
- a plurality of detectors;
- and
- means for directing the detected rays to the plurality of detectors so that the angle of incidence of the detected rays at the surface of secondary beam splitter is less than 20 degrees.
Dependencies are inferred from the claim text.