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US 7,746,552 B2Granted
Illumination module for evanescent illumination and microscope
AssigneeLeica Microsystems CMS GmbH
InventorsAndreas Hecker, Werner Knebel, Kyra Moellmann, Heinrich Ulrich
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Abstract
A microscope with a light source that produces an illumination light beam for evanescently illuminating a sample includes an adjustment mechanism with which the polarization of the illumination light beam may be changed.
Claims23
A microscope using a sample, comprising:
- an objective comprising an objective pupil,
- a light source generating an illumination light beam having a focus within the objective pupil and directed into the objective to produce evanescent illumination of the sample,
- a beam splitter in the illumination light beam splitting off a measuring beam from the illumination light beam, and
- a control mechanism controlling a depth of penetration of the evanescent illumination into the sample, comprising
- a polarization analyzer in the measuring beam determining polarization of the measuring beam and
- an adjustment mechanism changing polarization of the illumination light beam.
3 more elements
An illumination module for coupling to a microscope using a sample, the microscope having an objective pupil plane, the illumination module comprising;
- a light source generating an illuminating light beam having a focus in the objective pupil plane and producing evanescent illumination of the sample and
- a beam splitter in the illumination light beam splitting off a measuring beam from the illumination light beam, and
- a control mechanism controlling a depth of penetration of the evanescent illumination into the sample, comprising
- a polarization analyzer in the measuring beam determining polarization of the measuring beam and
- an adjustment mechanism changing polarization of the illumination light beam.
2 more elements
Dependencies are inferred from the claim text.