Bead for enantiomeric isomer resolution and process for producing the same

A process for producing a bead for enantiomeric isomer resolution comprising a polysaccharide derivative having a structure crosslinked with a crosslinking agent at a hydroxyl group that randomly remains at any of 2-, 3- and 6- positions of constituent units of the polysaccharide and the average number of remaining hydroxyl groups in all the constituent units of the polysaccharide derivative is one or less, the method comprising the steps of:

• reacting a derivative-forming compound in an amount required for converting 66 to 95% of all the hydroxyl groups contained in a polysaccharide into carbamate groups to thereby obtain a polysaccharide derivative;
• adding dropwise an organic solvent solution of the polysaccharide derivative to aqueous solution of a surfactant while being stirred to thereby produce beads;
• taking out the beads and, optionally, drying the beads after washing them;
• and
• reacting the beads with a crosslinking agent in the organic solvent to thereby react a hydroxyl group that randomly remains at any of the 2-, 3- and 6- positions in the constituent units of the polysaccharide with the crosslinking agent to thereby obtain a reaction mixture containing beads having a crosslinked structure.