Hexose oxidase-encoding DNAs and methods of use thereof
AssigneeDanisco A/S
InventorsPeter Stougaard, Ole Cai Hansen
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Abstract
A method of producing hexose oxidase by recombinant DNA technology, recombinant hexose oxidase and the use of such enzyme, in particular in the manufacturing of food products such as doughs and dairy products, animal feed, pharmaceuticals, cosmetics, dental care products and in the manufacturing of lactones. Suitable sources of DNA coding for the enzyme are marine algal species including Chondrus crispus, Iridophycus flaccidum and Euthora cristata. In useful embodiments, the recombinant hexose oxidase is produced by Pichia pastoris, Saccharomyces cerevisiae or E. coli.
Claims14
An isolated DNA fragment encoding a polypeptide having hexose oxidase activity, wherein said polypeptide oxidizes at least maltose and wherein said DNA fragment comprises a nucleic acid sequence isolated by hybridization with the nucleic acid sequence set forth in SEQ ID NO:
- 30 under stringent conditions comprising
- (1) hybridization at 65° C. for at least 14 hours in a buffer containing 1×Denhardt's solution, 2×SSC, 0.1% dextran sulphate, 50 μg/ml denatured salmon sperm DNA, followed by
- (2) two washes at 65° C. for 10 minutes in 2×SSC, 0.1% SDS, and followed by
- (3) two washes at 65° C. for 10 minutes in 1×SSC, 0.1% SDS.
Dependencies are inferred from the claim text.