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AssigneeJapan Science and Technology Agency
InventorsSeiji Shinkai, Takeshi Nagasaki, Takeshi Kawazu, Shinji Kakimoto
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To provide a new technique by which efficient transfection is ensured in delivering a target gene into a cell, disclosed is a nucleic acid construct for nuclear import, which comprises a ternary complex consisting of a nucleic acid substance containing a gene to be delivered into the nucleus of a cell, an importin protein (for example, importin-β) capable of passing through the nuclear pore and involved in the nuclear transport, and a binding substance (for example, polyethyleneimine) bound to both of the nucleic acid substance and the importin protein. Nucleic acid transport from outside of a cell into the cell nucleus can be particularly promoted by administering the nucleic acid construct bound to a cell membrane receptor binding factor and/or a membrane fusing substance, or administering the nucleic acid construct encapsulated in a non-viral vector (for example, Sendai virus envelope) having cell membrane permeability and membrane fusing properties.
A nucleic acid complex for nuclear import comprising a nucleic acid encoding a gene to be delivered into the nucleus of a cell, an importin protein capable of passing through a nuclear pore and involved in nuclear transport, and a polycationic binding substance bound to both said nucleic acid and said importin protein, wherein said nucleic acid is bound to said polycationic binding substance via a non-covalent bond such that the gene remains intact, and wherein the binding between said binding substance and said importin protein is via a biotin-avidin interaction in which the polycationic binding substance is biotinylated and bound to the importin protein via a biotin-avidin interaction.
A nucleic acid complex for nuclear import consisting of:
A nucleic acid complex for nuclear import comprising a) a nucleic acid encoding a gene to be delivered into the nucleus of a cell, b) an importin protein capable of passing through a nuclear pore and involved in nuclear transport, c) optionally, at least one of a cell membrane receptor binding factor and a membrane fusing substance, and d) a binding substance bound to said nucleic acid, said importin protein, and said at least one of the cell membrane receptor binding factor and the membrane fusing substance, where present, said nucleic acid being bound to said binding substance via a non-covalent bond such that the gene remains intact, the binding of said binding substance with said importin protein being via a covalent bond or non-covalent interaction, the binding of said binding substance with said at least one of the cell membrane receptor binding factor and the membrane fusing substance, where present, being via a covalent bond or non-covalent interaction, and said complex being encapsulated in an envelope or capsid of viral origin.
Dependencies are inferred from the claim text.