Cumate-inducible expression system for eukaryotic cells
AssigneeNational Research Council of Canada
InventorsBernard Massie, Alaka Mullick, Peter C. K. Lau, Yasuo Konishi
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Abstract
The invention relates to a new “gene-switch” (cumate-inducible switch) for mammalian cells. This switch is as useful in the development of expression systems and cell-based assays for functional genomics as in the generation of viral vectors for gene therapy.
Claims16
An isolated recombinant DNA molecule comprising:
- a) a mammalian promoter sequence having a TATA element;
- b) an operator sequence selected from the group consisting of P1 and P2 operator sequences positioned 3′ to the start of the transcription site, said P1 and P2 operator sequences consisting of SEQ ID NO:1 and SEQ ID NO:2, respectively;
- and
- c) the coding sequence of a gene lying 3′ to said operator and operably linked to said promoter.
A method for producing recombinant protein in a mammalian cell making the CymR repressor protein of Pseudomonas putida, said method comprising:
- a) transforming said mammalian cell in vitro with a vector comprising:
- (i) a mammalian promoter sequence having a TATA element;
- (ii) an operator sequence selected from the group consisting of P1 and P2 operator sequences positioned 3′ to the start of the transcription site, said P1 and P2 operator sequences consisting of SEQ ID NO:1 and SEQ ID NO:2, respectively;
- and
- (iii) the coding sequence of a gene lying 3′ to said operator and operably linked to said promoter wherein the coding sequence of said gene encodes said recombinant protein;
- b) introducing an effector molecule that regulates CymR-mediated expression into the transformed cells of step a) to induce the expression of said gene and produce said recombinant protein, said effector molecule being selected from the group consisting of p-ethylbenzoic acid, p-propylbenzoic acid, cumic acid, p-isobutylbenzoic acid, p-terbutylbenzoic acid, p-N-dimethylaminobenzoic acid, p-N-ethylaminobenzoic acid and cumate.
An isolated recombinantly engineered virus comprising within its genome:
- a) a recombinant promoter having a TATA element;
- b) an operator sequence selected from the group consisting of P1 and P2 operator sequences positioned 3′ to the start of the transcription site, said P1 and P2 operator sequences consisting of SEQ ID NO:1 and SEQ ID NO:2, respectively;
- and
- c) the coding sequence of a gene lying 3′ to said operator and operably linked to said promoter, wherein the coding sequence of said gene inhibits the replication of said virus when expressed.
An isolated recombinant DNA molecule comprising:
- a) a mammalian promoter sequence having a TATA element;
- b) at least one operator sequence selected from the group consisting of P1 and P2 operator sequences positioned 5′ to the TATA element, said P1 and P2 operator sequences consisting of SEQ ID NO:1 and SEQ ID NO:
- 2, respectively;
- and
- c) a gene lying 3′ to the TATA element and operably linked to the promoter.
An isolated recombinant DNA molecule comprising:
- a) a mammalian promoter;
- and
- b) the coding sequence of CymR-VP16 cumate activator operably linked to the promoter.
Dependencies are inferred from the claim text.