Antibodies and unnatural substrates of prenylation enzymes for use in detecting and isolating prenylated proteins
AssigneeUniversity of Kentucky Research Foundation
InventorsHans Peter Spielmann, Douglas A. Andres
Life of this patent
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Abstract
Unnatural substrates of prenylation enzymens and antibodies that recognize unique moieties of prenylated proteins, which unique moieties are transferred from the unnatural substrates are used for detecting and isolating prenylated proteins, and for screening for inhibitors of prenylation enzymes.
Claims10
An isolated antibody that recognizes a unique moiety of a protein modified therewith, the unique moiety being selected from the group consisting of:
- para-nitro-anilinogeranyl moiety, ortho-fluoro-anilinogeranyl moiety, para-cyano-anilinogeranyl moiety, para-nitro-anilinofarnesyl moiety, meta-cyano-anilinogeranyl moiety, and para-bromo-anilinogeranyl moiety, wherein the antibody that recognizes the unique moiety of the modified protein does not recognize farnesylated proteins or geranylgeranylated proteins.
An isolated antibody that recognizes a unique moiety of a protein modified therewith, the unique moiety being selected from the group consisting of:
- anilinogeranyl moiety, para-fluoro-anilinogeraniol moiety, meta-fluoro-anilinogeranyl moiety, para-methyl-anilinogeranyl moiety, meta-methyl-anilinogeranyl moiety, and meta-bromo-anilinogeranyl moiety, wherein the antibody that recognizes the unique moiety of the modified protein does not recognize farnesylated proteins or geranylgeranylated proteins.
An isolated monoclonal antibody that recognizes an anilinofarnesyl moiety of a protein modified therewith, wherein the antibody that recognizes the unique moiety of the modified protein does not recognize farnesylated proteins or geranylgeranylated proteins.
Dependencies are inferred from the claim text.