PH dependent adhesive peptides
AssigneeKansas State University Research Foundation
InventorsJohn Tomich, Takeo Iwamoto, Xinchun Shen, Xiuzhi Susan Sun
Life of this patent
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Abstract
A novel peptide adhesive motif is described that requires no receptor or cross-links to achieve maximal adhesive strength. Several peptides with different degrees of adhesive strength have been designed and synthesized using solid phase chemistries. All peptides contain a common hydrophobic core sequence flanked by positively or negatively charged amino acids sequences.
Claims6
An adhesive peptide selected from the group consisting of:
- E3h9E3 (SEQ ID NO:
- 4), K3h9E3 (SEQ ID NO:
- 5), E3h9K3 (SEQ ID NO:
- 6), K3h9K3 (SEQ ID NO:
- 7) and X3h9X3 (SEQ ID NO:
- 10),
- wherein
- h9 is the hydrophobic core sequence FLIVIGSII (SEQ ID NO:
- 2);
- K3 is the tripeptide lysine-lysine-lysine;
- E3 is the tripeptide glutamic acid-glutamic acid-glutamic acid;
- and
- X3 is the tripeptide diaminopropionic acid-diaminopropionic acid-diaminopropionic acid.
An adhesive peptide selected from the group consisting of:
- (K3h9)2K3 (SEQ ID NO:
- 8) and (K3h9)3K3 (SEQ ID NO:
- 9),
- wherein
- h9 is the hydrophobic core sequence FLIVIGSII (SEQ ID NO:
- 2);
- and
- K3 is the tripeptide lysine-lysine-lysine.
An adhesive peptide selected from the group consisting of:
- SEQ ID NOs:
- 25-29, 31 and 32.
A method comprising:
- a) providing:
- i) a peptide selected from the group consisting of SEQ ID NOs:
- 4-10, 25-29,31 and 32;
- ii) a solution having an approximate pH of 12;
- b) contacting said peptide with said solution under conditions that create an adhesive peptide.
A kit for the preparation of an adhesive peptide comprising:
- a syringe having a first barrel, said first barrel filled with an aqueous solution of a peptide selected from the group consisting of SEQ ID NOs:
- 4-10, 25-29, 31 and 32, buffered to maintain a pH between 6 and 7, and a second barrel, said second barrel filled with a base having a pH of approximately 12, wherein said first and second barrels are operably linked to a tip having a single lumen, wherein said lumen is in fluidic communication with said first and second barrels.
Dependencies are inferred from the claim text.