Synthesis and antimicrobial activity of novel dicationic “reversed amidines”

A pharmaceutical composition comprising a compound according to Formula I:

• wherein:
• R1, R2, R3 and R4 are each independently selected from the group consisting of H, alkyl, alkoxy, halide, and alkylhalide groups;
• R5 is H alkyl or aryl;
• R6 is aryl;
• and
• X is O;
• in a pharmaceutically acceptable carrier.

A method of treating an microbial infection in a subject in need of such treatment, wherein the microbial infection is caused by a microorganism selected from the group consisting of Mycobacterium tuberculosis, Trypanosoma spp., Candida albicans, Aspergillus spp., Cryptosporidium parvum, Giardia lamblia, Plasmodium spp., Pneumocystis carinii, Toxoplasma gondii, Fusarium solani, and Cryptococcus neoformans, said method comprising administering to the subject a compound according to Formula I or a pharmaceutically acceptable salt thereof:

• wherein:
• R1, R2, R3 and R4 are each independently selected from the group consisting of H, alkyl, alkoxy, halide, and alkylhalide groups;
• R5 is H, alkyl or aryl;
• R6 is aryl;
• and
• X is O.