Estrogen receptors and methods of use
AssigneeCreighton University
InventorZhao Yi Wang
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Abstract
The present invention provides isolated polypeptides having an amino acid sequence having at least 70% identity to SEQ ID NO:20, wherein the polypeptide has ER-α36 activity. The invention further provides methods for identifying agents that bind to such polypeptides, methods for detecting such polypeptides, and methods for altering the activity of such polypeptides. Also provided are antibodies that specifically bind to an amino acid sequence depicted at SEQ ID NO:1, or an immunogenic fragment thereof, and methods for making and using such antibodies.
Claims34
An isolated monoclonal antibody that specifically binds to an amino acid sequence depicted at SEQ ID NO:1.
A method for making an antibody comprising administering to an animal a polypeptide comprising an amino acid sequence depicted at amino acids 13-27 of SEQ ID NO:1 or an immunogenic fragment thereof, and isolating the antibody from the animal, wherein the isolated antibody specifically binds to amino acids 13-27 of SEQ ID NO:1.
A method for making an antibody comprising:
- administering to an animal a polypeptide comprising an amino acid sequence depicted at amino acids 13-27 of SEQ ID NO:1 or an immunogenic fragment thereof;
- obtaining from the animal a cell that produces the antibody;
- making a monoclonal antibody-producing hybridoma using the cell;
- and
- isolating a monoclonal antibody from the monoclonal antibody-producing hybridoma, wherein the isolated monoclonal antibody specifically binds to amino acids 13-27 of SEQ ID NO:1.
A method for detecting a polypeptide comprising:
- providing a cell;
- analyzing the cell for a polypeptide, wherein the polypeptide has a molecular weight of 36 kDa as measured following electrophoresis on a sodium dodecyl sulfate (SDS)-polyacrylamide gel, and is recognized by an isolated antibody that specifically binds to a polypeptide comprising the amino acid sequence depicted in SEQ ID NO:1;
- and
- determining whether the cell expresses the polypeptide;
- wherein analyzing the cell for a polypeptide comprises contacting the cell with an antibody that specifically binds to the amino acid sequence depicted at SEQ ID NO:1 or an immunogenic fragment thereof, and detecting the bound antibody.
Dependencies are inferred from the claim text.