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US 7,745,229 B2Granted
Chemoselective fluorgenic molecular linkers and methods for their preparation and use
AssigneeUniversity of South Carolina
InventorsQian Wang, Krishnamoorthy Sivakumar
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Abstract
The present invention describes a bioconjugation strategy and compounds that are useful therein in which a fluorescent signal is produced when two molecular or supramolecular entities are linked by chemoselective combination of one linker having an azido or halide substituent group with another linker having a cyano or an alkyne substituent group. A kit is also provided.
Claims40
A method of forming a linkage complex having a fluorescent signal, the method comprising:
- reacting a fluorophore with a linkage compound to form a linkage complex having a fluorescent signal that is different than a fluorescent signal of the fluorophore;
- wherein the fluorophore comprises coumarin substituted at position 3 or 4 with at least one azide group (—N3);
- and wherein the linkage compound has the structure
- where:
- m is 1 and LB comprises a linking group bonded to a biomolecule.
2 more elements
A kit for forming a linkage complex having a fluorescent signal, the kit comprising
- a fluorophore comprising coumarin substituted at position 3 or 4 with at least one azide group (—N3);
- and
- a linkage compound, wherein the linkage compound has the structure
- where:
- m is 1 and LB comprises a linking group bonded to a biomolecule.
2 more elements
Dependencies are inferred from the claim text.