Base sequence detection apparatus and base sequence automatic analyzing apparatus

An analyzing apparatus comprising:

• (a) a detection unit formed as a cassette and which detects a target base sequence in a sample solution on the basis of a reaction between the target base sequence in the sample solution and a probe base sequence, the detection unit including:
• a substrate on which a channel is formed, the sample solution, a first reagent solution, a second reagent solution, or air being supplied to the channel,
• a working electrode arranged in the channel with the probe base sequence immobilized thereon,
• a counter electrode which is arranged in the channel,
• a reference electrode which is arranged in the channel, and
• a sealing member which seals the channel on the substrate, which has inlet and outlet ports communicating with the channel;
• (b) a main apparatus on which the detection unit is attachably provided, the main apparatus including:
• a bypass provided in parallel with the channel of the detection unit, when the detection unit is attached to the main apparatus to detect the target base sequence;
• a supply unit including:
• a first supply valve which switches between supplying the first reagent solution and air to be supplied into the channel through the inlet port,
• a second supply valve which switches between supplying the second reagent solution and air to be supplied into the channel through the inlet port,
• a first nozzle attachably coupled to the inlet port and in communication with the inlet port when the detecting unit is attached to the main apparatus to detect the target base sequence, and
• a first valve, connected to the first and second supply valves and connected to the first nozzle and the bypass, to selectively supply one of the first and second reagent solutions and air into either one of the first nozzle or the bypass;
• a discharge unit including:
• a second nozzle attachably coupled to the outlet port and in communication with the outlet port when the detecting unit is attached to the main apparatus to detect the target base sequence,
• a second valve, connected to the second nozzle and the bypass, to selectively receive the reagent solution and air from either one of the second nozzle or the bypass, and
• a pump in communication with the second valve to suck the reagent
• solution and air to be discharged;
• the main apparatus further comprising:
• (i) a measurement unit which applies a voltage between the working electrode and the counter electrode and detects an electrochemical reaction signal from the working and the counter electrodes the measurement unit including:
• a voltage pattern generator which generates a voltage having a pattern,
• a feedback circuit which feeds back a voltage from the reference electrode,
• an amplifier which amplifies a voltage which is generated on the basis of the voltages from the voltage pattern generator and the feedback circuit, and applies the amplified voltage to the counter electrode, and
• (ii) a temperature control unit which controls a temperature of the detection unit;
• (iii) a control unit which controls the supply unit, the discharge unit, the measurement unit, and the temperature control unit;
• (iv) a memory unit which stores the electrochemical reaction signal as measurement data;
• and
• (v) a processing unit which processes the measurement data to determine the target base sequence.