Method of producing heterodimer derivative of protein phosphatase type 2A enzyme

A method for producing a catalytic subunit derivative of PP2A, comprising:

• infecting insect cultured cells with a baculovirus containing cDNA encoding the catalytic subunit of PP2A carrying a tag;
• incubating the infected cells at a temperature ranging from 16° C. to 22° C.;
• disrupting the incubated cells to obtain a disrupted cell suspension;
• and then
• purifying the disrupted cell suspension with a solid phase carrying a substance capable of binding to the tag, thus recovering a catalytic subunit derivative of PP2A.

A method for producing a phosphatase 2A (PP2A) protein heterodimer derivative that comprises the catalytic subunit of PP2A and the A subunit of PP2A, comprising:

• infecting cultured insect cells with baculovirus encoding
• a polypeptide comprising the catalytic subunit and a first tag and
• a polypeptide comprising the A subunit of phosphatase 2A protein and a second tag;
• incubating the infected cells at a temperature of from 16 to 22° C.;
• and
• recovering a phosphatase 2A protein heterodimer derivative from said cells.