Thermostable Y-family polymerases and chimeras
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsRoger Woodgate, John P. McDonald, Wei Yang
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Abstract
The present disclosure is related to thermostable Y-family polymerases, in particular several novel Y-family polymerases and chimeras made therefrom, as well as methods of identifying other Y-family polymerases, methods of generating other chimeric Y-family polymerases, methods of amplifying ancient or damaged DNA, and methods of incorporating fluorescent or modified nucleotides into a DNA molecule.
Claims8
An isolated chimeric Y-family polymerase comprising the amino acid sequence as set forth in
- SEQ ID NO:
- 57 (AiLFSte (AiDpo4/SteDpo4LF)) or a sequence having at least 95% sequence identity to SEQ ID NO:
- 57
- wherein the chimeric Y-family polymerase has polymerase activity.
A method of using a chimeric Y-family polymerase to add a nucleotide to a polynucleotide, the method comprising incubating a polynucleotide and a nucleotide with a chimeric Y-family polymerase, thereby adding the nucleotide to the polynucleotide, wherein the chimeric Y-family polymerase comprises SEQ ID NO:
- 57 (AiLFSte (AiDpo4/SteDpo4LF)).
Dependencies are inferred from the claim text.