Method for manufacturing xylitol with high-yield and high-productivity
AssigneesKorea Advanced Institute of Science and Technology, LPBIO Co., Ltd.
InventorsJung-Hoe Kim, Byoung-Sam Ko
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Abstract
The present invention provides a method for manufacturing xylitol with high-yield and high-productivity by using a xylitol dehydrogenase-deficient mutant of xylitol producing microorganism. This goal is achieved through modification of the metabolic pathway of the xylitol producing microorganism, preferably a natural xylose-assimilating yeasts and fungi, by disrupting or inactivating the expression of desired genes.
Claims7
A method for manufacturing xylitol which comprises a step of culturing a xylitol dehydrogenase-deficient mutant of Candida tropicalis whose xylitol dehydrogenase expression is disrupted by deletion, substitution or insertion into a part of the xylitol dehydrogenase coding sequence in a medium comprising xylose, a carbon source, a nitrogen source, and trace elements until the xylose contained in the medium is substantially consumed, whereby xylitol is produced.
Dependencies are inferred from the claim text.