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US 7,745,174 B2Granted
Hybrid hepatocyte growth factor gene having high expression efficiency of two heterotypes of hepatocyte growth factor
AssigneeViroMed Co., Ltd.
InventorsJong-Mook Kim, Woong Hahn, Eun-Jin Park
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Abstract
The present invention relates to a hybrid Hepatocyte Growth Factor (HGF) gene which is prepared by inserting an inherent or foreign intron between exons 4 and 5 in HGF cDNA, which has a base sequence of SEQ ID NO: 2. The gene has high expression efficiency and simultaneously expresses two heterotypes of HGF and dHGF (deleted variant HGF). Further the gene may be used for treating or preventing ischemic or liver diseases.
Claims21
A method for co-expressing two heterotypes of Hepatocyte Growth Factor (HGF) comprising transforming or transfecting to a cell a DNA construct comprising:
- (a) a promoter,
- (b) a first cDNA which has the same sequence as exons 1-4 of the human HGF gene wherein said exons 1-4 are arranged in sequential order without an intron therebetween, or degenerates thereof which do not alter the amino acid sequence encoded by said first cDNA,
- (c) a polynucleotide that has the same sequence as intron 4 of the HGF gene or a functional fragment thereof, and
- (d) a second cDNA which has the same sequence as exons 5-18 of the human HGF gene wherein said exons 5-18 are arranged in sequential order without an intron therebetween, or degenerates thereof which do not alter the amino acid sequence encoded by said second cDNA;
- wherein (c) is located between (b) and (d);
- and the HGF construct simultaneously encodes two heterotypes of human HGF.
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