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AssigneeShimadzu Corporation
InventorsToru Ezure, Shoken Higashide, Takashi Suzuki, Masaaki Ito, Koki Endo
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The present invention provides a preparation method of an insect cell extract solution for cell-free protein synthesis, the insect cell extract solution, a protein synthesis method in a cell-free system, which uses the insect cell extract solution, and a kit for cell-free protein synthesis containing the insect cell extract solution. The extract solution is easily prepared by the method of the present invention and can synthesize a higher amount of protein than by extract solutions prepared by conventional methods.
A method for cell-free protein synthesis, which comprises performing cell-free protein synthesis with an insect cell extract solution, wherein the insect cell extract solution is prepared by a process which comprises at least a step of rapid freezing at a temperature not higher than −80° C. an insect cell suspended in a solution for extraction, to allow for extraction from the insect cell, wherein the rapid freezing results in the insect cell being frozen for not longer than 10 seconds;
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