Method for identifying acetyltransferase substrates
AssigneeAlbert Einstein College of Medicine of Yeshiva University
InventorJohn S. Blanchard
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Abstract
The present invention is directed to a method of identifying an acetyltransferase substrate in a sample that includes the steps of: (a) contacting the sample with (i) a reagent comprising a thiol-containing compound, a halo-acetyl-CoA or a halo-acetyl-pantetheine, and (ii) an acetyltransferase, under conditions suitable for acetyltransferase enzyme activity, and (b) identifying a substrate that has formed a base-stable covalent bond to the reagent, wherein the reagent is labeled with a label and/or the acetyltransferase is labeled with an affinity tag, and the substrate is the acetyltransferase substrate.
Claims18
A method of identifying an acetyltransferase substrate in a sample, the method comprising the steps of:
- (a) contacting the sample with
- (i) a reagent comprising an aminoethanethiol, a halo-acetyl-CoA or a halo-acetyl-pantetheine, and
- (ii) an acetyltransferase,
- under conditions suitable for acetyltransferase enzyme activity, and
- (b) identifying a substrate that has formed a base-stable covalent bond to the reagent, wherein the reagent is labeled with a label and/or the acetyltransferase is labeled with an affinity tag, and the substrate is the acetyltransferase substrate.
Dependencies are inferred from the claim text.