Methods of determining binding between Nogo receptor and p75 neurotrophin receptor
AssigneeChildren's Medical Center Corporation
InventorsZhigang He, Kevin C. Wang, Jieun A. Kim
Life of this patent
Estimating the term…
Abstract
Inhibitors of Nogo Receptor (NgR)-p75 binding are used to reduce NgR-p75 binding mediated axon growth inhibition. Mixtures of NgR and p75 are used in pharmaceutical screens to characterize agents as inhibiting binding of NgR to p75 and promoting axon regeneration.
Claims21
A method for assaying Nogo receptor (NgR)-neurotrophin receptor p75NTR (p75) binding, the method comprising the steps of:
- providing a mixture comprising a p75-binding NgR domain and an NgR-binding p75 domain;
- and
- directly measuring binding of the NgR domain to the p75 domain as an indication of the assayed NgR-p75 binding.
A method for characterizing an agent as modulating Nogo receptor (NgR)-neurotrophin receptor p75NTR (p75) binding, the method comprising the steps of:
- A) incubating a mixture comprising a p75-binding NgR domain and an NgR-binding p75 domain under conditions that allow for binding between the two domains and directly measuring the degree of binding between the two domains, and
- B) incubating a mixture comprising a p75-binding NgR domain, an NgR-binding p75 domain, and an agent under the same conditions as in A), and directly measuring the degree of binding between the two domains;
- wherein a difference between the binding measured in A) and the binding measured in B) indicates that the agent modulates NgR-p75 binding.
A method for assaying Nogo receptor (NgR)-neurotrophin receptor p75NTR (p75) binding, the method comprising the steps of:
- recombinantly expressing a p75-binding NgR domain and an NgR-binding p75 domain;
- and
- measuring binding of the NgR domain to the p75 domain.
Dependencies are inferred from the claim text.