Method for stabilization of proteins in solution

A method for preparing and stabilizing one or more analytes in solutions for a denaturing immunoassay of body samples, comprising the steps of:

• i) obtaining a solubilized mammalian body sample containing one or more analytes in a sample medium comprising a denaturing agent of SDS at 0.1-1% w/v, wherein the one or more analytes are selected from the group consisting of p16INK4a, EpCAM, gCatenin, MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7;
• ii) subsequently heating said sample medium containing the one or more analytes to a temperature of 90-110 ±2° C. for a period of time of 3 to 45 minutes;
• iii) stabilizing and storing the one or more heated analytes in said sample medium, and
• iv) subsequently performing a denaturing immunoassay on said sample medium containing the one or more analytes and the denaturing agent of SDS.