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US 7,745,142 B2Granted
Molecular modification assays
AssigneeMolecular Devices, LLC
InventorsAnnegret Boge, Luke D. Lavis, J. Richard Sportsman, Merl F. Hoekstra, Wei Huang
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Abstract
Assays for detecting molecular modifications such as phosphate modifications and the presence and/or activity of enzymes and other agents involved in facilitating or otherwise regulating such modifications.
Claims25
A method of detecting nucleotide cyclization or decyclization in a sample, comprising:
- contacting a nucleotide with a binding partner that binds specifically to a noncyclized form but not to a cyclized form of the nucleotide, the binding partner including at least one metal ion required for specific binding of the binding partner to the noncyclized form of the nucleotide;
- contacting the nucleotide with at least one enzyme selected from the group consisting of cyclases and phosphodiesterases;
- detecting a response indicative of the extent of binding between the noncyclized form of the nucleotide and the binding partner;
- and
- correlating the response with a cyclization or decyclization activity of the at least enzyme on the nucleotide.
2 more elements
Dependencies are inferred from the claim text.