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US 7,745,135 B2Granted
Method for detecting reaction product of nucleic acid synthesis
AssigneeEiken Kagaku Kabushiki Kaisya
InventorsYasuyoshi Mori, Kentaro Nagamine
Life of this patent
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Abstract
A method for detecting the occurrence of nucleic acid syntheses using an enzyme through the use of a generated insoluble substance as an indicator.
Claims6
A kit for detecting the occurrence of nucleic acid amplification or for monitoring nucleic acid amplification comprising the following elements:
- (a) when a first arbitrary sequence F1c, a second arbitrary sequence F2c, and a third arbitrary sequence F3c are selected in that order from the 3′ terminus in a target region toward the 3′ terminus on the polynucleotide chain, and a fourth arbitrary sequence R1, a fifth arbitrary sequence R2, and a sixth arbitrary sequence R3 are selected in that order from the 5′ terminus in the target region toward the 5′ terminus of the nucleotide chain,
- a primer containing sequence F2 which is complementary to F2c and, on the 5′ side of F2, the same sequence as F1c;
- a primer containing sequence F3 which is complementary to F3c;
- a primer containing the same sequence as R2 and, on the 5′ side of the sequence, sequence R1c which is complementary to R1;
- and
- a primer containing the same sequence as R3;
- (b) a polymerase catalyzing strand displacement-type synthesis of complementary chain;
- (c) a nucleotide serving as a substrate for the element (b);
- (d) a melting temperature regulator;
- and
- (e) a coagulant.
8 more elements
A kit for detecting the occurrence of nucleic acid amplification or for monitoring nucleic acid amplification comprising the following elements:
- (a) when a first arbitrary sequence F1c and a second arbitrary sequence F2c are selected in that order from the 3′ terminus in a target region toward the 3′ terminus on the polynucleotide chain and a third arbitrary sequence R1 and a fourth arbitrary sequence R2 are selected in that order from the 5′ terminus in the target region toward the 5′ terminus of the nucleotide chain,
- a primer containing sequence F2 which is complementary to F2c and, on the 5′ side of F2, the same sequence as F1c;
- and
- a primer containing the same sequence as R2and, on the 5′ side of the sequence, sequence R1c which is complementary to R1;
- (b) a polymerase catalyzing strand displacement-type synthesis of complementary chain;
- (c) a nucleotide serving as a substrate for the element (b);
- (d) a melting temperature regulator;
- and
- (e) a coagulant.
6 more elements
Dependencies are inferred from the claim text.