Prognostic indicators of canine lymphoid neoplasia using tumor-derived plasma DNA
AssigneeThe Ohio State University
InventorLaura J. Rush
Life of this patent
Estimating the term…
Abstract
A method of identifying an animal having a disease or condition without clinical symptoms includes detecting an elevated level of plasma DNA from blood or a blood fraction from the animal. In particular, the disease or condition can be a lymphoid neoplastic disease. Also, the plasma DNA can comprise a tumor-derived plasma DNA. In certain embodiments, the evaluation of the blood or blood fraction from the animal for the mutation assists in the identification of the disease or condition and/or its prognosis in the animal.
Claims13
A method of evaluating the disease condition of a canine suspected of having or at risk of having a lymphoid neoplasia-related disease, the method comprising:
- (i) obtaining a sample of plasma from the canine suspected of having or at risk of having the lymphoid neoplasia-related disease;
- (ii) detecting the presence of plasma nucleic acid in the sample;
- and
- (iii) identifying the canine as having or at risk of having the lymphoid neoplasia-related disease when the level of the detected plasma nucleic acid is 25 ng/ml or greater.
A method for detecting a lymphoid neoplasia-related disease in a canine, the method comprising the steps of:
- (i) purifying extracellular nucleic acid from blood plasma from the canine to prepare extracted extracellular nucleic acid, and concurrently or sequentially,
- (ii) amplifying extracellular nucleic acid or a fragment thereof, or amplifying a signal from the extracellular nucleic acid or a fragment thereof;
- and
- (iii) detecting the product of the amplified extracellular nucleic acid or the product of its amplified fragment, or the amplified signal of the extracellular nucleic acid or the amplified signal of a fragment thereof;
- wherein the extracellular nucleic acid comprises a DNA encoding a mutated gene or fragment thereof;
- (iv) identifying the canine as having lymphoid neoplasia when 25 ng/ml or greater of the mutated gene or fragment thereof is detected.
A method of identifying a canine having a lymphoid neoplastic disease without clinical symptoms comprising
- detecting an elevated level of 25 ng/ml or greater of extracellular nucleic acid from plasma DNA from the canine
- wherein the plasma DNA comprises a tumor-derived plasma DNA.
Dependencies are inferred from the claim text.