Ras mutation and compositions and methods related thereto
AssigneeGlobeImmune, Inc.
InventorsZhimin Guo, Yingnian Lu, Donald Bellgrau, Alex Franzusoff
Life of this patent
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Abstract
Disclosed are newly discovered Ras mutations and combinations of mutations, proteins and peptides and fusion proteins containing these mutations, nucleic acid molecules encoding such proteins, peptides, and fusion proteins, and a variety of tools and diagnostic, therapeutic, and screening methods associated with the use of such mutations.
Claims15
A method for detecting mutated Ras in a test sample, comprising:
- a. obtaining a test sample from a patient;
- and
- b. detecting whether the test sample contains cells expressing a mutant Ras having a substitution of a glycine, a lysine, or a glutamine for the glutamate at position 76 with respect to the wild-type Ras amino acid sequence.
A method for detecting ras having a mutation at codon 76 in a patient tumor sample, comprising:
- a) isolating genomic DNA from a patient tumor sample;
- b) amplifying a DNA fragment from the genomic DNA using oligonucleotide PCR primers that flank a ras nucleic acid sequence that includes nucleotides encoding the amino acid at position 76 of Ras;
- and
- c) probing the amplified sequence with oligonucleotides that hybridize specifically with sequences containing one or more mutations at codon 76 of ras, but not with wild-type ras sequences, to detect the presence of a nucleic acid sequence encoding Ras having a substitution of a glycine, a lysine, or a glutamine for the glutamate at position 76.
A method for detecting ras encoding a Ras protein having a mutation of the amino acid at position 76 in a patient tumor sample, comprising:
- a) obtaining a tumor sample from a patient;
- and
- b) performing a hybridization and/or amplification reaction on said tumor sample under conditions such that one determines whether the tumor sample comprises ras encoding a Ras protein having a substitution of a glycine, a lysine, or a glutamine for the glutamate at position 76.
Dependencies are inferred from the claim text.