Hybridization method
AssigneeEisai R&D Management Co., Ltd.
InventorsChikako Hakii, Mitsugu Usui
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Abstract
A hybridization method is provided in which an efficient hybridization reaction can be carried out. Further, there are provided, using this hybridization method, a method for detecting a target gene with high sensitivity and a signal amplifying method for markedly improving the detection sensitivity of the target gene. There is provided a hybridization method comprising the use of oligonucleotides in a reaction solution, the method comprising forming partially a reaction temperature region in the reaction solution and performing a hybridization reaction in the reaction temperature region.
Claims32
A hybridization method comprising,
- performing a hybridization reaction in a reaction solution, wherein the hybridization reaction is a self-assembly reaction forming a double-stranded self-assembly substance by hybridizing plural kinds of oligonucleotide probes having complementary base sequence regions hybridizable to each other to self-assemble the oligonucleotide probes, and
- wherein the reaction solution has a high temperature region and a low temperature region and the hybridization reaction preferentially occurs in the high temperature region.
Dependencies are inferred from the claim text.