Back to results
US 7,745,123 B2Granted
Cell cycle reporting cell line
AssigneesGe Healthcare UK Limited, Vanderbilt University
InventorsSuzanne Hancock, Simon Stubbs, Nicholas Thomas, Ellen Fanning, Jinming Gu
Life of this patent
Estimating the term…
Abstract
The present invention relates to non-destructive and dynamic means for determining the cell cycle position of living cells. The invention provides stable cell lines which can be used to determine the cell cycle position, together with methods for measuring the effect of a test agent on the cell cycle position.
Claims8
A stable cell line expressing:
- i) a first polypeptide construct comprising a first detectable live-cell reporter molecule linked to at least one cell cycle phase-dependent location control element that is a cell cycle phase position motif which is the phosphorylation-dependent subcellular localization domain of the C-terminal special control region of helicase B (PSLD), wherein the location is indicative of the cell cycle position;
- and
- ii) a second polypeptide construct comprising a second detectable live cell reporter molecule such as an enzyme or fluorescent protein linked to a destruction control element that is the cyclin B1 D box, wherein said second reporter is detectable in a mammalian cell at a predetermined position in the cell cycle;
- wherein said first and second reporter molecules are chosen to be distinguishable.
1 more element
Dependencies are inferred from the claim text.