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AssigneeIndustrial Technology Research Institute
InventorsWen-Tyng Li, Rung-Jiun Gau, Yu-Shih Weng, Cheng-Ta Hsieh, Pei-Shan Li
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The present invention provides a cell-culturing model and a method for screening compounds which can be applied in treating or preventing hepatic cirrhosis. The cell culturing model comprises a population of hepatocytes and hepatic stellate cells (HSCs) derived from co-culturing, and at least one population of the cells comprises a nucleotide sequence fragment of a reporter gene and a cell specific regulatory sequence. The cell-culturing model of the present invention can be applied in high throughput screening for effective compounds of medication, and also in understanding the functional mechanism of the effective compounds.
A cell culturing model comprising co-cultured human hepatocytes and human hepatic stellate cells (HSCs) having a cell specific regulatory sequence operatively linked to a heterologous reporter gene nucleotide sequence, and wherein said cell specific regulatory sequence is a human hepatic stellate cell specific promoter comprising a collagen type 1 promoter having the nucleotide sequences selected from the group consisting of SEQ ID NO. 14 and SEQ ID NO. 15.
A cell culturing model comprising co-cultured human hepatocytes and human hepatic stellate cells (HSCS) a cell specific regulatory sequence and a nucleotide sequence fragment of a reporter gene, wherein the cell specific regulatory sequence is a hepatocyte specific promoter or a hepatic stellate cell specific promoter having a sequence selected from the group consisting of SEQ ID NO:
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