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US 7,745,105 B2Granted
Method of preparing soft tissue for a biological scaffold by lyophilizing, heating and elastase treatment
AssigneeJapan Health Sciences Foundation
InventorsToshiya Fujisato, Dohiko Terada, Kazuya Sawada, Takeshi Nakatani
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Abstract
A method for preparing a scaffold material for use in the prosthesis therapy is disclosed. The method comprises (a) lyophilizing a segment of native soft tissue of mammalian origin, heating the lyophilized tissue at a temperature of 100-200° C., and incubating the tissue with elastase to selectively remove elastin leaving the extracellular components mainly comprised of collagen.
Claims10
A method for preparing a scaffold material for use in the prosthesis therapy comprising the steps of:
- (a) lyophilizing a segment of native soft tissue of mammalian origin;
- (b) heating the lyophilized tissue under vacuum at a temperature between 100° C. and 200° C. whereby partial crosslinking and fixation of proteins take place;
- and
- (c) incubating the tissue with elastase to selectively remove elastin leaving extracellular components mainly comprised of collagen.
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Dependencies are inferred from the claim text.