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US 7,744,921 B2Granted
Liposome loading with metal ions
AssigneeCelator Pharmaceuticals, Inc.
InventorsPaul Tardi, Sharon Johnstone, Murray Webb, Marcel Bally, Sheela Abraham
Life of this patent
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Abstract
This invention relates to encapsulation of drugs and other agents into liposomes.
Claims10
A method of loading at least one therapeutic agent into a liposome in a liposome composition, the method comprising:
- i) providing a composition comprising liposomes, said liposomes lacking an ionophore, in an external solution, the liposomes containing an internal copper-ion compatible solution comprising encapsulated copper ions;
- ii) adding at least one therapeutic agent to the external solution;
- and
- iii) maintaining the agent in the external solution for sufficient time to load the agent into the liposomes, whereby said copper or cobalt ions effect said loading;
- wherein the external solution and external surfaces of the liposome contain substantially no uncomplexed copper ions;
- and
- wherein the liposomes comprise 0-20 mol % cholesterol.
4 more elements
A method of loading at least one therapeutic agent into a liposome in a liposome composition, the method comprising:
- i) providing a composition comprising liposomes in an external solution, the liposomes containing an internal copper-ion compatible solution comprising encapsulated copper ions;
- ii) adding at least one therapeutic agent to the external solution;
- and
- iii) maintaining the agent in the external solution for sufficient time to load the agent into the liposomes,
- wherein the external solution and external surfaces of the liposome contain substantially no uncomplexed copper ions;
- and
- wherein the liposomes comprise 0-20 mol % cholesterol;
- and
- wherein the liposomes do not at any time during said loading comprise an ionophore, whereby said copper ions effect said loading.
6 more elements
A method of loading at least one therapeutic agent into a liposome in a liposome composition, the method comprising:
- i) providing a composition comprising liposomes in an external solution, the liposomes containing an internal copper-ion compatible solution comprising encapsulated copper ions;
- ii) adding at least one therapeutic agent to the external solution;
- and
- iii) maintaining the agent in the external solution for sufficient time to load the agent into the liposomes,
- wherein the external solution and external surfaces of the liposome contain substantially no uncomplexed copper ions;
- and
- wherein the liposomes comprise 0-20 mol % cholesterol;
- and
- wherein said loading is conducted in the absence of ionophore, whereby the therapeutic agent is encapsulated at levels greater than those achieved in the absence of said copper ions.
6 more elements
Dependencies are inferred from the claim text.