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US 7,655,787 B2Granted
pRNA chimera
AssigneePurdue Research Foundation
InventorsPeixuan Guo, Stephen M. Hoeprich, Dan Shu
Life of this patent
Estimating the term…
Abstract
A circularly permuted chimeric pRNA molecule carrying a stabilized biologically active RNA, such as a ribozyme.
Claims18
A pRNA chimera comprising:
- (a) a pRNA region;
- and
- (b) a spacer region comprising a biologically active RNA, the spacer region covalently linked at its 5′ and 3′ ends to the pRNA region, wherein the biologically active RNA specifically binds a pre-identified substrate;
- wherein the pRNA region comprises:
- (i) in the 5′ to 3′ direction beginning at the covalent linkage of the pRNA with the 3′ end of the spacer region
- a first loop;
- a second loop;
- and
- a lower stem-loop structure comprising a bulge, a first stem section and a third loop;
- (ii) a second stem section interposed between the spacer region and the stem-loop structure;
- (iii) a third stem section interposed between the stem-loop structure and the first loop;
- (iv) a fourth stem section interposed between the first loop and the second loop;
- and further comprises
- (v) an opening defining 5′ and 3′ ends of the pRNA chimera.
11 more elements
A pRNA chimera comprising:
- (a) a pRNA region;
- and
- (b) a spacer region comprising a biologically active RNA that specifically binds a pre-identified ligand or receptor, the spacer region covalently linked at its 5′ and 3′ ends to the pRNA region;
- wherein the pRNA region comprises:
- (i) in the 5′ and 3′ direction beginning at the covalent linkage of the pRNA with the 3′ end of the spacer region
- a first loop;
- a second loop;
- and
- a lower stem-loop structure comprising a bulge, a first stem section and a third loop;
- (ii) a second stem section interposed between the spacer region and the stem-loop structure;
- (iii) a third stem section interposed between the stem-loop structure and the first loop;
- (iv) a fourth stem section interposed between the first loop and the second loop;
- and further comprises
- (v) an opening defining 5′ and 3′ ends of the pRNA chimera.
11 more elements
A pRNA chimera comprising:
- (a) a pRNA region;
- and
- (b) a spacer region comprising a biologically active RNA that comprises at least one of a specific binding activity and an enzymatic activity, the spacer region covalently linked at its 5′ and 3′ ends to the pRNA region;
- wherein the pRNA region comprises:
- (i) in the 5′ and 3′ direction beginning at the covalent linkage of the pRNA with the 3′ end of the spacer region
- a first loop;
- a second loop;
- and
- a lower stem-loop structure comprising a bulge, a first stem section and a third loop;
- (ii) a second stem section interposed between the spacer region and the stem-loop structure;
- (iii) a third stem section interposed between the stem-loop structure and the first loop;
- (iv) a fourth stem section interposed between the first loop and the second loop;
- and further comprises
- (v) an opening defining 5′ and 3′ ends of the pRNA chimera.
11 more elements
A pRNA chimera comprising:
- (a) a pRNA region;
- and
- (b) a spacer region comprising a biologically active RNA that does not comprise the nucleotide sequence UAAUACGACUCACUAUA as set forth in SEQ ID NO:8, the spacer region covalently linked at its 5′ and 3′ ends to the pRNA region, wherein the biologically active RNA specifically binds a pre-identified substrate;
- wherein the pRNA region comprises:
- (i) in the 5′ to 3′ direction beginning at the covalent linkage of the pRNA with the 3′ end of the spacer region
- a first loop;
- a second loop;
- and
- a lower stem-loop structure comprising a bulge, a first stem section and a third loop;
- (ii) a second stem section interposed between the spacer region and the stem-loop structure;
- (iii) a third stem section interposed between the stem-loop structure and the first loop;
- (iv) a fourth stem section interposed between the first loop and the second loop;
- and further comprises
- (v) an opening defining 5′ and 3′ ends of the pRNA chimera.
11 more elements
A pRNA chimera comprising:
- (a) a pRNA region;
- and
- (b) a spacer region comprising a biologically active RNA, the spacer region covalently linked at its 5′ and 3′ ends to the pRNA region, wherein the biologically active RNA binds a pre-identified substrate and comprises a polyribonucleotide selected from the group consisting of an RNA of at least 25-100, 46, 47, 63, 66, 94, 120, 168 and 188 nucleotides,
- wherein the pRNA region comprises:
- (i) in the 5′ to 3′ direction beginning at the covalent linkage of the pRNA with the 3′ end of the spacer region
- a first loop;
- a second loop;
- and
- a lower stem-loop structure comprising a bulge, a first stem section and a third loop;
- (ii) a second stem section interposed between the spacer region and the stem-loop structure;
- (iii) a third stem section interposed between the stem-loop structure and the first loop;
- (iv) a fourth stem section interposed between the first loop and the second loop;
- and further comprises
- (v) an opening defining 5′ and 3′ ends of the pRNA chimera.
11 more elements
Dependencies are inferred from the claim text.